3D全自动外泌体荧光检测分析系统

3D全自动外泌体荧光检测分析系统


法国Abbelight公司开发的3D全自动外泌体荧光检测分析系统是一款无需纯化的、全自动的可对单个外泌体进行表征分析的全新设备。该设备能够提供全方位的外泌体表征信息,包括外泌体粒径大小、亚型分布、携带蛋白表达、单个外泌体的膜蛋白与生物标志物共定位等。操作简单,结果可靠。

3D全自动外泌体荧光检测分析系统基于特异性免疫捕获技术,允许研究者直接分析特定群体的外泌体。通过单分子定位技术成像,可以得到单个外泌体的超分辨成像结果,尺度可以到20nm全自动外泌体荧光检测分析系统兼容各种生物样本,除了纯化的外泌体之外,对于血液、尿液、恶性肿瘤、腹水中的外泌体也可直接检测分析,大大拓展了研究范围。


通过单分子定位技术成像,可以得到单个外泌体的超分辨成像结果,尺度可以到20nm,对于血液、尿液、恶性肿瘤、腹水中的外泌体也可直接检测分析。#

应用方向及主要特征


无须纯化

特异性捕获

超分辨成像与粒径统计

表面蛋白与内容物分析
多通道成像
单外泌体共定位分析


超高分辨单外泌体成像

左:2D单个外泌体成像;右:3D单个外泌体成像


外泌体粒径分析:通过团簇分析外泌体的整体和亚群的粒径分布


膜蛋白表征与共定位分析,以CD63,CD81&CD9为例表征其单阳,双阳和三阳的比例


外泌体内容物表征

对比穿膜处理后外泌体中内容物aGFP的表达 


观测细胞中外泌体的分布情况,研究细胞中细胞器或者其他蛋白和外泌体的定位关系


应用案例

法国Abbelight公司开发的3D全自动外泌体荧光检测分析系统是一款无需纯化的、全自动的可对单个外泌体进行表征分析的全新设备。该设备能够提供全方位的单个外泌体表征信息,包括外泌体粒径大小、亚型分布、携带蛋白表达、单个外泌体的膜蛋白与生物标志物共定位等。

肿瘤诊断

载药系统开发

眼科疾病诊断

疫苗研发

脊髓受伤机制研究

血浆/血清外泌体分析

外泌体工程化

呼吸疾病诊断

外泌体2D成像


外泌体3D成像


 

外泌体形态分析

 


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