3D单分子荧光成像系统-SAFe 360

3D单分子荧光成像系统-SAFe 360


SAFe 360是法国abbelight公司推出的一款基于单分子定位的显微成像(SMLM)的3D单分子成像系统,它独有的DAISY技术整合了散光技术和超临界角光技术,能够极大的提高定位精度,达到最高的xyz同时15nm的定位精度,可以提供清晰度最高的三维的亚细胞结构图像,最高同时四色成像观测,可以实时研究不同的结构功能蛋白的共定位信息,在单分子水平研究分子动力学反应以及细胞间的相互作用等。


加装
TIRF
PALM
STORM
SPT

smFRET

...... 


兼容
Confocal
Spinning-Desk
Widefield
SIM

STED

......


先进的DAISY技术具备高性能、低漂移、四色同时成像等优点

设备参数


+ 成像模式:PALM、STORM、PAINT、smFRET 、SPT

+ 光源模式:Epi、TIRF、HILO

+ 最高分辨率:15 nm的XYZ轴分辨率

+ 超大视野:200 × 200 μm2的视野

+  一次可同时采集1.2 μm深度图像信息

+  最高图像深度:10 μm

+  实时漂移矫正

+  最高四色同时成像

+  活细胞成像模式


配套试剂


Smart kit


•  10 doses per box

•  200 µL per dose

•  30 sec prepartion

•  2 months in a fridge

•  2 weeks on sample



Compatible dyes


•  Atto 488, WGA-AF®488

•  AF®532, CF®532, Cy3b

•  AF®555, AF®594, CF®555, AF®568, CF®568, Cy5, MemBriteTM 568, TMR

•  AF®647, CF®647, AF®680, CF®680, MemBriteTM 640, Actin-stain 670, SiR647



3D线粒体结构


核孔复合物


老鼠海马神经元


微管蛋白网络




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[3] Belkahla, Hanen, et al. "Carbon dots, a powerful non-toxic support for bioimaging by fluorescence nanoscopy and eradication of bacteria by photothermia." Nanoscale Advances (2019).

[4] Denis, Kevin, et al. "Targeting Type IV pili as an antivirulence strategy against invasive meningococcal disease." Nature microbiology 4.6 (2019): 972.

[5] Szabo, Quentin, et al. "TADs are 3D structural units of higher-order chromosome organization in Drosophila." Science advances 4.2 (2018): eaar8082. 

[6] Boudjemaa, Rym, et al. "Impact of bacterial membrane fatty acid composition on the failure of daptomycin to kill Staphylococcus aureus." Antimicrobial agents and chemotherapy 62.7 (2018): e00023-18.

[7] Culley, Siân, et al. "Quantitative mapping and minimization of super-resolution optical imaging artifacts." Nature methods 15.4 (2018): 263.

[8] Berger, Stephen L., et al. "Localized myosin II activity regulates assembly and plasticity of the axon initial segment." Neuron 97.3 (2018): 555-570.

[9] Cabriel, Clément, et al. "Aberration-accounting calibration for 3D single-molecule localization microscopy." Optics letters 43.2 (2018): 174-177. 

[10] Bouissou, Anaïs, et al. "Podosome force generation machinery: a local balance between protrusion at the core and traction at the ring." ACS nano 11.4 (2017): 4028-4040. 

[11] Sellés, Julien, et al. "Nuclear pore complex plasticity during developmental process as revealed by super-resolution microscopy." Scientific reports 7.1 (2017): 14732.

[12] Bourg, Nicolas, et al. "Direct optical nanoscopy with axially localized detection." Nature Photonics 9.9 (2015): 587. 

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