ExoView外泌体全面表征试剂盒
ExoView外泌体全面表征试剂盒
ExoView外泌体全面表征试剂盒

ExoView外泌体全面表征试剂盒


外泌体计数、粒径、蛋白表达、蛋白共定位一次完成


检测样本类型

对细胞培养上清、血浆、血清、尿液、脑脊液、唾液等生物样本中的外泌体直接进行分析

捕获抗体种类

anti-CD81, anti-CD9, anti-CD63, 同型IgG对照;可自定义

单次上样体积

35 µl稀释样本

重复检测数目

3复孔

荧光抗体种类

CD9(Blue)CD81(Green)CD63(Red)


实验原理


① 35 μL外泌体样品滴加在芯片上孵育;

② 预先包被的抗体特异结合外泌体表面蛋白以捕获外泌体;

③ 再使用荧光抗体特异性标记需要表征的标记物;

④ 最后用ExoView R100检测外泌体粒径、计数、蛋白表达(CD9,CD81,CD63等)及共定位。



检测流程


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产品类别


产品货号产品名称
EV-TETRA-C人外泌体检测试剂盒
EV-TETRA-P人血浆外泌体检测试剂盒
EV-TETRA-M2鼠外泌体检测试剂盒
EV-TETRA-C-CAR人外泌体内容物检测试剂盒
EV-TETRA-P-CAR人血浆外泌体内容物检测试剂盒
EV-TC-FLEX自由捕获人外泌体检测试剂盒
EV-TP-FLEX自由捕获人血浆外泌体检测试剂盒
EV-TC-FLEX-CAR自由捕获人外泌体内容物检测试剂盒
EV-TP-FLEX-CAR自由捕获人血浆外泌体内容物检测试剂盒
EV-TM-FLEX自由捕获鼠外泌体检测试剂盒
EV-TM-FLEX-CAR自由捕获鼠外泌体内容物检测试剂盒
EV-FLEX-2自由捕获外泌体检测试剂盒
EV-FLEX-2 -CAR自由捕获外泌体内容物检测试剂盒
EV-CTETRA-1/2/3人外泌体检测试剂盒+1/2/3个自定义捕获抗体
EV-CTETRA-1/2/3-CAR人外泌体内容物检测试剂盒+1/2/3个自定义捕获抗体
EV-CUST-1/2/3/4/5/6自定义1/2/3/4/5/6抗体捕获外泌体检测试剂盒
EV-CUST-1/2/3/4/5/6-CAR自定义1/2/3/4/5/6抗体捕获外泌体内容物检测试剂盒



试剂盒特点


特异性捕获

芯片上可包被多达6种捕获抗体,特异性捕获含特定蛋白标记物的外泌体。


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绝对阳性外泌体计数

芯片捕获外泌体后,可通过SP-IRIS技术直接检测样品中外泌体的数量。



单个外泌体蛋白共定位分析

检测每个外泌体的荧光信号并进行统计,可获得荧光共定位信息,用于分析样品中不同表型外泌体的比例(如右图所示)



无需纯化

使用抗体捕获模式,防止样品中杂质影响结果,可直接检测血液、尿液和细胞培养液中的外泌体,未纯化样品的测量结果与纯化后基本一致(如右图所示)



粒径分辨率高

高精度SP-IRIS技术,可检测≥50 nm的外泌体,测量结果与电子显微镜检测结果基本一致,并统计生成外泌体的粒径分布结果(如右图所示)



可检测外泌体内容物

试剂盒配套相应的穿膜剂,可穿透外泌体并对外泌体内容物进行染色并检测,未穿膜时只能检测到跨膜蛋白CD9的荧光信号,穿膜后即可检测到外泌体内容物Syntenin的表达(如右图所示)






外泌体荧光数量统计


外泌体粒径检测


外泌体内容物检测


荧光强度与粒径关系


荧光共定位分析




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