■  单分子定位技术研究线粒体

线粒体是一种存在于大多数细胞中的由两层膜包被的细胞器结构，大部分线粒体尺寸小于1微米，是细胞制造能量的细胞器，还参与细胞分化，增殖代谢等诸多生理学过程。同时线粒体是对各种损伤为敏感的细胞器之一，细胞损伤常见的病理改变可概括为线粒体数量、大小和结构的改变等。通过研究线粒体结构与功能的变可以研究各种细胞损伤以及不同线粒体疾病。通过单分子定位（SMLM）技术可以精确观测线粒体的三维结构和形态，研究特定蛋白表达以及药物处理后诸如线粒体肿大等形态结构的变化， 观测线粒体的融合与分裂以及衰老对于这一过程的影响等。

1. 线粒体三维结构观测

2. 特定蛋白表达与否对线粒体形态变化影响

左右分别为不同蛋白表达后线粒体的形态，不同的蛋白表达后线粒体的宽度不同

3. 小分子药物对于线粒体融合的影响

图A为未经过药物小分子处理的线粒体，B为年老的细胞经过小分子处理后的线粒体，C为年轻的细胞经过小分子处理后的线粒体。直观对比可以发现，此小分子有助于线粒体的融合，同时年轻的细胞的融合效果好于年老的细胞

[1] Cabriel, Clément, et al. "Combining 3D single molecule localization strategies for reproducible bioimaging." Nature communications 10.1 (2019): 1980.

[2] Woodhams, Stephen G., et al. "Cell type–specific super-resolution imaging reveals an increase in calcium-permeable AMPA receptors at spinal peptidergic terminals as an anatomical correlate of inflammatory pain." Pain 160.11 (2019): 2641-2650.

[3] Belkahla, Hanen, et al. "Carbon dots, a powerful non-toxic support for bioimaging by fluorescence nanoscopy and eradication of bacteria by photothermia." Nanoscale Advances (2019).

[4] Denis, Kevin, et al. "Targeting Type IV pili as an antivirulence strategy against invasive meningococcal disease." Nature microbiology 4.6 (2019): 972.

[5] Szabo, Quentin, et al. "TADs are 3D structural units of higher-order chromosome organization in Drosophila." Science advances 4.2 (2018): eaar8082. 

[6] Boudjemaa, Rym, et al. "Impact of bacterial membrane fatty acid composition on the failure of daptomycin to kill Staphylococcus aureus." Antimicrobial agents and chemotherapy 62.7 (2018): e00023-18.

[7] Culley, Siân, et al. "Quantitative mapping and minimization of super-resolution optical imaging artifacts." Nature methods 15.4 (2018): 263.

[8] Berger, Stephen L., et al. "Localized myosin II activity regulates assembly and plasticity of the axon initial segment." Neuron 97.3 (2018): 555-570.

[9] Cabriel, Clément, et al. "Aberration-accounting calibration for 3D single-molecule localization microscopy." Optics letters 43.2 (2018): 174-177. 

[10] Bouissou, Anaïs, et al. "Podosome force generation machinery: a local balance between protrusion at the core and traction at the ring." ACS nano 11.4 (2017): 4028-4040. 

[11] Sellés, Julien, et al. "Nuclear pore complex plasticity during developmental process as revealed by super-resolution microscopy." Scientific reports 7.1 (2017): 14732.

[12] Bourg, Nicolas, et al. "Direct optical nanoscopy with axially localized detection." Nature Photonics 9.9 (2015): 587. 

3D单分子荧光成像系统-SAFe 360.pdf

1分钟快速了解大视野单分子超分辨模块