外泌体计数、粒径、蛋白表达、蛋白共定位一次完成
检测样本类型 | 对细胞培养上清、血浆、血清、尿液、脑脊液、唾液等生物样本中的外泌体直接进行分析 |
捕获抗体种类 | anti-CD81, anti-CD9, anti-CD63, 同型IgG对照；可自定义 |
单次上样体积 | 35 µl稀释样本 |
重复检测数目 | 3复孔 |
荧光抗体种类 | CD9（Blue）/ CD81（Green）/ CD63（Red） |
无须纯化即可直接对血浆、血清、尿液、培养基中的外泌体进行表征的新方法。该设备能偶提供单个外泌体水平下的全方位外泌体表征信息，包括了颗粒大小、计数、表型与生物标志物共定位等。
实验原理
① 35 μL外泌体样品滴加在芯片上孵育；
② 预先包被的抗体特异结合外泌体表面蛋白以捕获外泌体；
③ 再使用荧光抗体特异性标记需要表征的标记物；
④ 后用ExoView R100检测外泌体粒径、计数、蛋白表达（CD9，CD81，CD63等）及共定位。
检测流程
产品类别
| 产品货号 | 产品名称 |
| EV-TETRA-C | 人外泌体检测试剂盒 |
| EV-TETRA-P | 人血浆外泌体检测试剂盒 |
| EV-TETRA-M2 | 鼠外泌体检测试剂盒 |
| EV-TETRA-C-CAR | 人外泌体内容物检测试剂盒 |
| EV-TETRA-P-CAR | 人血浆外泌体内容物检测试剂盒 |
| EV-TC-FLEX | 自由捕获人外泌体检测试剂盒 |
| EV-TP-FLEX | 自由捕获人血浆外泌体检测试剂盒 |
| EV-TC-FLEX-CAR | 自由捕获人外泌体内容物检测试剂盒 |
| EV-TP-FLEX-CAR | 自由捕获人血浆外泌体内容物检测试剂盒 |
| EV-TM-FLEX | 自由捕获鼠外泌体检测试剂盒 |
| EV-TM-FLEX-CAR | 自由捕获鼠外泌体内容物检测试剂盒 |
| EV-FLEX-2 | 自由捕获外泌体检测试剂盒 |
| EV-FLEX-2 -CAR | 自由捕获外泌体内容物检测试剂盒 |
| EV-CTETRA-1/2/3 | 人外泌体检测试剂盒+1/2/3个自定义捕获抗体 |
| EV-CTETRA-1/2/3-CAR | 人外泌体内容物检测试剂盒+1/2/3个自定义捕获抗体 |
| EV-CUST-1/2/3/4/5/6 | 自定义1/2/3/4/5/6抗体捕获外泌体检测试剂盒 |
| EV-CUST-1/2/3/4/5/6-CAR | 自定义1/2/3/4/5/6抗体捕获外泌体内容物检测试剂盒 |
试剂盒特点
特异性捕获 芯片上可包被多达6种捕获抗体，特异性捕获含特定蛋白标记物的外泌体。 |
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阳性外泌体计数 芯片捕获外泌体后，可通过SP-IRIS技术直接检测样品中外泌体的数量。 |
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单个外泌体蛋白共定位分析 检测每个外泌体的荧光信号并进行统计，可获得荧光共定位信息，用于分析样品中不同表型外泌体的比例（如右图所示）。 |
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无需纯化 使用抗体捕获模式，防止样品中杂质影响结果，可直接检测血液、尿液和细胞培养液中的外泌体，未纯化样品的测量结果与纯化后基本一致（如右图所示）。 |
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粒径分辨率高 高精度SP-IRIS技术，可检测≥50 nm的外泌体，测量结果与电子显微镜检测结果基本一致，并统计生成外泌体的粒径分布结果（如右图所示）。 |
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可检测外泌体内容物 试剂盒配套相应的穿膜剂，可穿透外泌体并对外泌体内容物进行染色并检测，未穿膜时只能检测到跨膜蛋白CD9的荧光信号，穿膜后即可检测到外泌体内容物Syntenin的表达（如右图所示）。 |
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外泌体荧光数量统计
外泌体粒径检测
外泌体内容物检测
荧光强度与粒径关系
荧光共定位分析
中国用户已发表文章
☛ 南京大学李靓/张辰宇/张玉婧课题组在《Frontiers in Cell and Developmental Biology》发表文章。
☛ 上海大学在《Journal of extracellular vesicles》发表文章。
☛ 中国科学院深圳技术研究院在《Lab on a Chip》发表文章。
☛ 北京天坛医院、国家纳米科学中心、北京航空航天大学在《Advanced Science》发表文章。
☛ 同济大学附属上海市肺科医院、上海思路迪转化医学在《Journal of Nanobiotechnology》发表文章。
☛ 山东千佛山医院在《NANO LETTERS》发表文章
感谢用户老师对Quantum Design China & NanoView设备的认可与支持！
2022-2023发表文章
• Richard J. R. Kelwick …& Paul S. Freemont. (2023) Opportunities to accelerate extracellular vesicle research with cell-free synthetic biology. Journal of Extracellular Vesicles.
• Laurence Blavier …& Yves A. DeClerck. (2023) The capture of extracellular vesicles endogenously released by xenotransplanted tumours induces an inflammatory reaction in the premetastatic niche. Journal of Extracellular Vesicles.
• Hongyun Wang ……& Junjie Xiao. (2022) Extracellular vesicles enclosed-miR-421 suppresses air pollution (PM2.5)-induced cardiac dysfunction via ACE2 signalling. Journal of Extracellular Vesicles.
• Linglei Jiang……& Santosh Dhakal. (2022) A7 bacterial extracellular vesicle-based intranasal vaccine against SARS-CoV-2 protects against disease and elicits neutralizing antibodies to wild-type and Delta variants. Journal of extracellular vesicles.
• Shaobo Ruan, Nina Erwin, Mei He. (2022) Light-induced high-efficient cellular production of immune functional extracellular vesicles. Journal of extracellular vesicles.
• Nasibeh Karimi ……& Cecilia Lässer. (2022) Tetraspanins distinguish separate extracellular vesicle subpopulations in human serum and plasma – Contributions of platelet extracellular vesicles in plasma samples. Journal of Extracellular Vesicles.
• Heikki Kyykallio ……& Pia R-M Siljander. (2022) A quick pipeline for the isolation of 3D cell culture-derived extracellular vesicles. Journal of Extracellular Vesicles.
• Roberto Frigerio ……& Marina Cretich. (2022) Comparing digital detection platforms in high sensitivity immune-phenotyping of extracellular vesicles. Journal of Extracellular Vesicles.
• Yael Hirschberg ……& Inge Mertens. (2022) Characterizing extracellular vesicles from individual low volume cerebrospinal fluid samples, isolated by SmartSEC. Journal of Extracellular Vesicles.
• Sukhbir Kaur……& David D. Roberts. (2022) Single vesicle analysis of CD47 association with integrins and tetraspanins on extracellular vesicles released by T lymphoblast and prostate carcinoma cells. Journal of Extracellular Vesicles.
• Simone M. Crivelli……& Erhard Bieberich. (2022) Function of ceramide transfer protein for biogenesis andsphingolipid composition of extracellular vesicles. Journal of Extracellular Vesicles.
• Gi Beom Kim …& In-San Kim. (2023) Harnessing Oncolytic Extracellular Vesicles for Tumor Cell-Preferential Cytoplasmic Delivery of Misfolded Proteins for Cancer Immunotherapy. Small.
• J. Klein, Y. Cao. (2023) Multi-Lipid Synergy In Cartilage Lubrication: Redundancy Or Evolution? Osteoarthritis and Cartilage.
Valerie DeLuca …& Michael E. Berens. (2023) Extracellular vesicles as a pharmacodynamic reporter in glioblastoma. Cancer Research.
• Sarah Pike …& Liya Xu. (2023) Extracellular vesicles in diagnostic retinoblastoma aqueous humor correlate with disease stage and clinical outcome: a pilot study. Cancer Research.
• D. Cookson …& Y. Ashraf Kharaz. (2023) The Biological Response Of Anterior Cruciate Ligamentocytes Following Treatment With Synovial Fluid Extracellular Vesicles. Osteoarthritis and Cartilage.
• Mei Lu …& Yuanyu Huang. (2023) Antitumor synergism between PAK4 silencing and immunogenic phototherapy of engineered extracellular vesicles. Acta Pharmaceutica Sinica B.
• Luke C. McIlvenna ……& Martin Whitham. (2023) Single vesicle analysis reveals the release of tetraspanin positive extracellular vesicles into circulation with high intensity intermittent exercise. The Journal of Physiology.
• Joseph Blommer ……& Dimitrios Kapogiannis. (2023) Extracellular vesicle biomarkers for cognitive impairment in Parkinson’s disease. BRAIN.
• Tyler J ……& Atta Behfar. (2022) Exosome biopotentiated hydrogel restores damaged skeletal muscle in a porcine model of stress urinary incontinence. Npj Regenerative Medicine.
• Min Han ……& Tao Xin. (2022) Three-Dimensional-Cultured MSC-Derived Exosome-Hydrogel Hybrid Microneedle Array Patch for Spinal Cord Repair. Nano Letters.
• Zijian Yang ……& David A. Issadore. (2022) Ultrasensitive Single Extracellular Vesicle Detection Using High Throughput Droplet Digital Enzyme-Linked Immunosorbent Assay. Nano Letters.
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ExoView外泌体荧光检测分析系统 产品资料
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纳米颗粒追踪分析（NTA）与ExoView的重要对比
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ExoVoew外泌体全面表征试剂盒
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优化的共定位分析
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