模块化超分辨共聚焦显微系统-LiveCodim
模块化超分辨共聚焦显微系统-LiveCodim

模块化超分辨共聚焦显微系统-LiveCodim



传统荧光显微镜受到光学衍射极限的影响,最高的分辨率为200 nm,因此很难观察细胞中的超微结构。LiveCodim是一款模块化超分辨共聚焦显微系统,能够适配绝大多数的倒置荧光显微镜,将现有的倒置显微镜升级成为具备宽场、共聚焦、超分辨三大模式的成像系统。LiveCodim通过独特的锥形衍射显微镜—— 一种强大的波束成形器,能够直接提供分辨率高达120 nm的实时活细胞超分辨共聚焦成像,同时无需对样品进行任何额外操作,结合其低光毒性,以及方便快捷的操作系统等优势,是拍摄荧光成像的不二之选。

可以进行x,y,z,时间序列和多通道的活细胞超分辨成像,可以实时观测诸如细胞器动态变化,小分子转运,以及细胞分裂等非常精密的动力学过程

产品优势


·  超高性价比:模块化超分辨,节省成本,兼容绝大多数倒置显微镜

·  xy轴超高分辨率:<120 nm

·  z轴深度成像:具备z-stack成像能力,最高成像深度50 μm

·  活细胞成像:低光毒性和光漂白性,适合活细胞成像

·  制样简单:样品无需特殊处理,无需特殊染料

·  全自动软件:全自动调节各种参数,简单易上手


主要参数


·  xy轴分辨率:< 120 nm

·  z轴分辨率:< 500 nm

·  z轴成像深度:50 μm

·  成像视野:共聚焦模式下80 μm * 80 μm,超分辨模式下: 50 μm * 50 μm

·  成像模式:宽场、共聚焦、LiveCodim超分辨

·  四色成像通道:405 nm, 488 nm, 561 nm, 640 nm (根据需求可增加)


1.  MDCK细胞中线粒体的动态变化




2.  Hela胞的微管宽场,共聚焦,LiveCodim超分辨成像



3.  细胞分裂中期的COS-7细胞3D多色超分辨成像




4.  植物细胞成像:观测铃兰草的根茎


5.  天然免疫分子TRIM5α作用机制研究

天然免疫分子TRIM5α蛋白是人类基因中决定疾病的易感性和发病速度的重要因素,其抗病毒活性通常通过小泛素相关修饰物(SUMO)调节,但是具体的作用机制仍有待进一步研究。LiveCodim超分辨图像揭示了TRIM5α主要分布在肌小管的核膜上,同时与存在于核孔的细胞质丝上的RanGTPase激活蛋白RanGAP1有明显的共定位现象,和主要定位于核篮上的蛋白Nup153无明显共定位,说明TRIM5α主要定位于这类细胞的胞质面。


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法国巴斯德研究所


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蒙彼利埃大学